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JU INSIGHT: Staining-free, Automated Sperm Analysis for In Vitro Fertilization Lab Use

By: Changsheng Dai, PhD; Guanqiao Shan, PhD; Sahar Jahangiri, MS; Wenyuan Chen, MS; Banafshe Gholamhoseini, PhD; Zhuoran Zhang, PhD; Sergey Moskovtsev, MD; Clifford Librach, MD; Keith Jarvi, MD; Yu Sun, PhD | Posted on: 01 Dec 2022

Dai C, Shan G, Jahangiri S, et al. Staining-free, automated sperm analysis for in vitro fertilization lab use. J Urol. 2022;208(6):1303-1312.

Study Need and Importance

Figure. Staining-free, automated system to perform quantitative sperm analysis on live samples. A, Sperm analyzer setup. B, A field of view with each sperm automatically tracked and labeled.

Computer-aided sperm analysis is typically used in andrology labs, not in in vitro fertilization (IVF) labs, which requires staining for sperm morphology measurement. In IVF labs, sperm analysis still relies on manual observation and suffers from subjectivity and inconsistency.

What We Found

We developed a staining-free, automated sperm analysis system as shown in the Figure, possessing high reproducibility and reliability. Automated measurement of concentration, motility, and morphology had intraclass correlation coefficients higher than 0.97. The regression and Bland-Altman analysis indicated that automated measurement and off-line manual benchmarking with zoomed-in images were interchangeable. High reproducibility and reliability were also attained in further analysis on semen and washed samples and the measurement on progressive and nonprogressive motility.

Limitations

A limitation of the developed sperm analyzer lies in its inability to measure subcellular parameters such as acrosome and vacuole, due to the low imaging contrast without staining.

Interpretation for Patient Care

Our staining-free, automated system is designed for routine use in IVF labs to perform quantitative sperm analysis on live samples. Automated and accurate sperm measurement can help improve patient care in infertility treatment.

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